<div dir="ltr">Logistically how will this work and is this email chain different from the other one? I've been pretty swamped with other obligations lately, but am very interested in this pre-ordering and testing one.</div><div class="gmail_extra"><br><div class="gmail_quote">On Mon, Jun 13, 2016 at 1:13 PM, Keoni Gandall <span dir="ltr"><<a href="mailto:koeng101@gmail.com" target="_blank">koeng101@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">Looks great! I am excited to test the machine out. </div><div class="gmail_extra"><br><div class="gmail_quote"><div><div class="h5">On Mon, Jun 13, 2016 at 2:13 PM, John Griessen <span dir="ltr"><<a href="mailto:john@industromatic.com" target="_blank">john@industromatic.com</a>></span> wrote:<br></div></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div><div class="h5">Thanks to Bryan Bishop I will be developing an electroporator<br>
as in the attached proposal starting Wednesday, (and a little setting up<br>
Q&A with suppliers today). Here's the feature list from the proposal:<br>
<br>
Programming will be via USB device endpoint for serial ports, and referencing micropython documentation, plus a quick how-to published on <a href="http://kitmatic.com" rel="noreferrer" target="_blank">kitmatic.com</a> under the product docs section.<br>
<br>
Power will be by 28 Volt “wall wart” external UL listed power supply.<br>
<br>
Output zaps will be disabled when USB is plugged in – no USB remote controlling, (safety reasons).<br>
<br>
Programmability of output zaps will be by a sequence of python commands that do not guarantee a definite pulse shape. Pulse shape will have to be arrived at by trial and error, using external observations with oscilloscopes to a voltage divided down test point that is inherently not safe to touch, and the whole oscilloscope is not safe when using it to observe the HV discharges. The reason it is not safe is the voltage divider chain of resistors could fail conductive-→oscilloscope gets zapped-→people touching oscilloscope usually.<br>
<br>
Micropython running on a STM32F030CCT6 which costs only $1.30 or STM32F401CBU6 which costs $2.8 will power the sequence and control functions.<br>
<br>
Indicator LEDs and pushbuttons will be provided for Ready, High, Medium and Low states of the machine. The Ready LED will be red or green and when red, pushing Ready button “gets it ready” for the next zap. High, Medium and Low pushbuttons change to different stored zap profiles named High, Medium and Low.<br>
<br>
Fire button causes a discharge.<br>
<br>
Batt indicator LED is low duty cycle blinking red when battery needs charging soon.<br>
<br>
Standard electroporation cuvettes with aluminum contacts will be connected somehow to the zap output such that high voltage cannot jump a gap to to where the cuvette mounts when a safety guard is removed for loading and unloading cuvettes by hands. The safety guard lid will probably operate like a knife switch where contacts, are more than 7mm apart and are bridged by a copper strip, (or pogo pins), when the lid is pushed on. When the lid is open, no bare contacts will have high voltage on them. Bare metal with HV might be present inside a 3mm hole that a pogo pin goes into, or inside a 1.5mm insulated slot that a copper strip goes into to make contact. Fingers randomly touching the cuvette area would not touch anything but insulator and have an air gap to HV of more than 7mm.<br>
<br>
The overall size will be less than 10cm X 10cm X 5cm tall if using electroporation cuvettes, and smaller if using pipectrodes.<br>
local LiFePO4 battery, circuits to charge it, detect state of charge.<br>
<br>
Flyback switching power supply with flyback 57:1 transformer to generate at least 3kV into a 30kOhm load starting from the low 3.2V of the LiFePO4 battery, or the 28VDC external power supply.<br>
<br>
28VDC external power supply (from AC 120V). Either/or a 28VDC external power supply (from AC 240V).<span><font color="#888888"><br>
<br>
John Griessen<br>
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