[Open_electroporator] Introduction

John Griessen john at industromatic.com
Wed Mar 12 21:56:38 UTC 2014


On 03/12/2014 12:02 PM, NeonJohn wrote:
>> 1. to clean up the pulses should just be an LC filter, and maybe a
>> >load resistor, right (and maybe a feedback ADC)?
> You'd need a diode to prevent the charge from each pulse discharging
> back through the coil.  I have a data sheet on some 17kV diodes but
> they're only rated at 50 ma.
>
>> >Hmm, I'm not sure I understand what you mean. I 'interested in the
>> >driver' that is cheap, available, and fits the circuit/application. I
>> >know there are some things called coils and transformers (and gosh
>> >I've even heard of autotransformers) but couldn't really say what they
>> >were or what to look for in a part for this. All I really know is the
>> >turn ratio for transformers, and that I need a HV transformer, and HV
>> >silicon switches I think, and maybe HV capacitor (but I think the
>> >transformer replaces the cap).
> What I need to know is how much voltage and current you need for
> electroporation?  Tell me that and I'll tell you how to get there.  If
> you can wait a few days until I get finished with my Fluxeon work I'll
> design it for you.


Here's a flyback with microcontrollers idea for doing pulses from previous discussion.
Two pulsers could be used alternating to generate a ripply steady
HV if they can take the heat.

http://ecosensory.com/diybio/flyback-porator-pulser-1.png
http://ecosensory.com/diybio/flyback-porator-pulser-2.png
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