[Open_electroporator] recommenadtions after John Griessen's lame pulser design ideas

TQ Hirsch thequux at thequux.com
Wed Mar 19 00:45:25 UTC 2014


On Fri, Mar 14, 2014 at 10:32 AM, John Griessen <john at industromatic.com>wrote:

> On 03/14/2014 11:39 AM, NeonJohn wrote:
>
>>
>> I think this is a case where a microprocessor is inappropriate.  One can
>> spend months duplicating in an algorithm what a few discrete components
>> and a dedicated chip can do.
>>
>
> The problem is the algorithm is subject to change and having a sequence
> of pulses you can program is better than having a steady ripply output.
> Experiments are one shot, and will just cook the live critters if left on
> too long.  So some programability is really desired.
>

 It is also an option, presuming that ~20ns resolution is acceptable and
you can afford $3-4 and some board space, to build what is essentially a
single-bit DDS: hook a microcontroller to an SRAM, have the microcontroller
write a 1-bit PCM signal to be output to the SRAM and then play it back
with discrete logic or a CPLD (It would be a few 74-series chips plus the
µC and SRAM). For bonus points, it doesn't have to be a 1-bit DAC. You can
build an 8-16 bit DAC out of discretes fairly easily (presuming that you
have a HV switch at your disposal), and thus have the world's only
programamble waveform (not just programmable pulse) electroporator.

--tq
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