[Open_electroporator] crude electroporator testing

[William Beeson]

Thanks for taking this on. I have everything in my lab necessary to test on
E.coli and fungi. My suggestion is to start with E.coli because it's very
easy to transform and we will be able to tell if the basic design has a
chance of working.

Below was the lowest cost electroporation device I could find new, but they
didn't know if it would work on fungi.

http://www.tritechresearch.com/CG-2.html
On May 1, 2016 2:27 AM, "John Griessen" <john at industromatic.com> wrote:

> On 05/01/2016 12:04 AM, Keoni Gandall wrote:
> > Just make sure if you send one that there are lots and lots of safety
> instructions included (I only have basic safety knowledge
> > due to not being able to take any classes on electronics).
>
> Oh, yes. It will be marked.  Maybe I'll figure a way to put a safety
> switch on yours too! :-)
>
> The idea of a safety switch is it disables the danger when the lid is open
> to expose the wires
> that contact the cuvette electrodes.  I could put some kind of hinged lid
> with a limit switch
> that closes when the lid is down and weighted down enough to push the
> switch.
>
> Then the test procedure would be:
> 1. load cuvette with xx volume of sample
> 2. measure resistance of loaded cuvette with Fluke DVM.
> 3. put cuvette in clothespin holder rig up.
> 4. close lid and add rock on top.
> 5. issue commands at python interpreter command window to setup duration
> and number of pulses.
> 6. issue python command to fire.
>
> Without a safety switch, the commands would be:
> 1. unplug wall wart AC-2-DC converter.
> 2. load cuvette with xx volume of sample
> 3. measure resistance of loaded cuvette with Fluke DVM.
> 4. be sure 10 seconds has elapsed since unplugging.
> 5. put cuvette in clothespin holder rig up.
> 6. close lid and add rock on top.
> 7. plug wall wart AC-2-DC converter into electroporator.
> 7. issue commands at python interpreter command window to setup duration
> and number of pulses.
> 8. issue python command to fire.
>
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