[Open_electroporator] list is back with slight name change

[Keoni Gandall]

That's true. A 2000$ price tag also isn't a viable solution for DIYbio,
hopefully that could be lowered.

I have not, I'm still hacking together a way to use it / make comp cells.
Will update when I try it

On Fri, May 27, 2016 at 3:11 PM, William Beeson <beesonwt at gmail.com> wrote:

> Used equipment isn't a viable solution for wide scale use. Interested to
> hear how the 400 one works? You tried it yet?
> On May 27, 2016 9:00 AM, "Keoni Gandall" <koeng101 at gmail.com> wrote:
>
>> I recently got a used gene pusler, full set, for about 400$ off eBay.
>> It's going to be hard to beat that. I also got "the electroporator from
>> invitrogen" for 42$ (can't cancel bids) so I am gonna try that out as well.
>>
>> I don't know if I can afford 2000 for an electroporator, but I can test
>> cells on these used ones to see if it's much better.
>>
>> -Keoni
>>
>> Sent from my iPhone
>>
>> On May 27, 2016, at 4:44 AM, William Beeson <beesonwt at gmail.com> wrote:
>>
>> Price is listed on the site. About 2000 for voltage adjustable
>> bactozapper.
>> On May 27, 2016 12:45 AM, "Nathan McCorkle" <nmz787 at gmail.com> wrote:
>>
>>> Did you hear a price?
>>>
>>> On Thu, May 26, 2016 at 8:48 PM, William Beeson <beesonwt at gmail.com>
>>> wrote:
>>> > All,
>>> >
>>> > Today I spoke on the phone with Andrew Papp of Tritech Research.  He
>>> is the
>>> > inventor of the bactozapper cloning gun.  I think he invented it back
>>> in
>>> > 1993 and his company builds them in house.  He was very nice and
>>> > knowledgeable about every aspect of how it was built.   I told him how
>>> there
>>> > was a DIY community that would probably be interested in these devices
>>> if
>>> > they could be sold at a lower price or marketed to them more
>>> specifically.
>>> > His company isn't very big on marketing, but this is currently the
>>> cheapest
>>> > type of electroporator and actually appears to be better than cuvettes
>>> from
>>> > an ease of use perspective. I think the patent has expired
>>> > (https://patents.google.com/patent/US5422272A/en).  Unfortunately
>>> they are
>>> > sold out of the bactozapper and he said it may take as long as a month
>>> for
>>> > them to build another batch.  I am pretty sure I'm going to place an
>>> order
>>> > for the adjustable voltage version of the bactozapper.
>>> >
>>> > -Will
>>> >
>>> > http://www.tritechresearch.com/aboutus.html
>>> >
>>> >
>>> >
>>> > On Sun, May 22, 2016 at 11:58 PM, John Griessen <
>>> john at industromatic.com>
>>> > wrote:
>>> >>
>>> >> No news, just recovered from server fail,
>>> >> and new server is upgradeable, so less likely to go away.
>>> >> The old one was based on openvz, which keeps same old kernel if
>>> provider
>>> >> is lazy,
>>> >> and mine was just milking my account till death.  I triggered the
>>> death
>>> >> accidentally
>>> >> trying to upgrade it partially, but went too far and had kernel
>>> >> dependencies that
>>> >> ended it silently.
>>> >>
>>> >> JG
>>> >> _______________________________________________
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>>> >
>>> >
>>> >
>>> > _______________________________________________
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>>> >
>>>
>>>
>>>
>>> --
>>> -Nathan
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