[Open_electroporator] low cost bacterial non-salty electroporation zappers

Fri May 27 20:27:11 UTC 2016

Stationary as in the organism life/reproductive cycle? Seems more like
electrical settings than cuvette or equipment magic.

As a semi-professional electrical engineer who has been interested and
focused on high-performance analog electronics for lab equipment since my
first year of undergrad in biotechnology... I feel like John's internet
presence indicates fairly high capability to achieve the goal according to
his timeline. Whether $2000 is easily half to a quarter the price any
day-job professional or consultant would make on a similar job in
industrial (or corporate sales oriented) setting. A senior engineer in
high-ish energy arbitrary signal waveform generation would probably be
another double of the highest cost I just estimated. So I think that means
John is highly motivated, or highly confident. His price estimates seem
right on as well. A monitor and computer rarely cost $1000-2000 anymore...
I just bought a $30 Android phone and $80 2-in-1 tablet/laptop (with Intel
Inside!) (Of course higher end machines certainly exist into the multi
thousand dollar range, but these are no longer the entry-point or even
average ).

If the product John proposes was hackable enough to support normal bio lab
scientist use, as well as my use cases (testing arbitrary cuvettes, such as
capillary or microfluidic channels)... then I'd be interested in some kind
of kickstarter per-purchase type deal (if I pitch in $1K now, I get 5-10
working gizmos sooner than later).
On May 27, 2016 12:58 PM, "William Beeson" <beesonwt at gmail.com> wrote:

> I don't think there is any issue using the pipettes with other
> electroporation equipment. I'm not interested in the business of selling
> hardware, I just want a reasonably priced, reasonably reliable way to do
> electroporation. Andrew was telling me on the phone his system even works
> with stationary cells really well and that just dramatically simplifies the
> whole process of making competent cells.
>
> I will test it and buy it, maybe a few of them, if it works.
> On May 27, 2016 3:17 PM, "John Griessen" <john at industromatic.com> wrote:
>
>> On 05/27/2016 01:21 PM, William Beeson wrote:
>>
>>> The concept is to make a device that is compatible with the pipectrodes
>>> that tritech sells, but at a cost of $250?
>>>
>>
>> No, selling price.  Costs to produce would be electronics $22,
>> electronics assembly $10, plastics $15, final assembly $35
>> testing $20 shipping/kitting $15  profit $133.
>>
>> The kit version selling at $95 would be electronics $22, electronics
>> assembly $10, plastics $15, testing $20 shipping/kitting $15, profit
>>  $13.  (Well maybe some early birds at that price, then $125 kits.)
>>
>> On 05/27/2016 01:23 PM, William Beeson wrote:
>> > He indicated they were building a lot of microscopes and incubators. I
>> was pretty frustrated that there was no way to buy a
>> > bactozapper. He actually suggested trying to find one on eBay.
>>
>>
>> Sounds like they must be piddly for him and his employees.  He's right
>> about ebay, I saw a $450 price for one.
>> Fastest way.
>>
>> quick-result, low-price, high-quality: pick any two...
>>
>> Are you interested in buying copy two of my first kludge version of
>> electroporator?  Using his pipectrodes
>> for experimenting should be easy after talking with him, just ask to buy
>> some, (which are labeled in stock on his site).
>>
>> If you think he is not agreeable to selling them without his machines,
>> maybe just lie that you found one to buy second hand and
>> need some.  50 could be years of use if you wash them.
>>
>> Searching for them planetwide, you find Young-ho, Lee (Biomaterials
>> Pacific Co., Ltd.)  I've asked what they sell for...
>> Maybe it is expired patent and available from the asian factory direct
>> now...
>>
>>
>>
>> Biomaterials Pacific Co., Ltd.
>> #402 Sundo B/D, 96 Yanjae-dong Seocho-gu, Seoul Korea
>>     Products/Services:cloning gun, pipectrode, palm system, molecular
>> biology, immunology
>>
>>     Business Type:Manufacturer
>>
>>     Year Established:1999
>>
>>     Employees Total:101 - 500
>>
>> Maybe no need to lie.  They list cloning gun and pipectrode as
>> products....  Hmmm....
>>
>> John
>>
>> =========================Tritech Research Inc 2961 Veteran Ave Los
>> Angeles============================
>> Revenue:        $1 mil. - $5 mil.
>>         $2,400,000
>> Employees: 10 - 20
>> Industry:  Biotechnology , Pharmaceuticals , Healthcare
>> Company Description: Tritech Research was started by Andrew Papp, Ph.D.
>> (Harvard University, 1989) a molecular biologist frustrated with
>> over-priced, often poorly designed research equipment that can make bench
>> science tedious and unnecessarily time consuming. All the products we offer
>> are designed to promote research. Some items do this by saving time so
>> researchers can get more done, while others save them money. Each product
>> we offer has been designed and tested under laboratory conditions with
>> utility, convenience, quality and economy in mind. When customers call to
>> place an order or for customer service, their questions are answered by a
>> skilled research biologist who can explain all the features (and any
>> limitations) of our products. We gladly recommend solutions even if they
>> involve products from other companies. We are open to, and appreciate,
>> suggestions on how we can improve our products or service, as well as to
>> new product ideas. We can even design custom equipment for unique research
>> needs at reasonable prices. Click HERE to learn more about our Custom
>> Engineering Solutions.
>>
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