crude electroporator testing

William Beeson beesonwt at gmail.com
Sun May 1 15:50:46 UTC 2016


Here is a full overview of this type of product. It looks pretty simple and
is much less expensive than the big desktop ones.

http://www.tritechresearch.com/clongun.html
On May 1, 2016 11:46 AM, "William Beeson" <beesonwt at gmail.com> wrote:

> Thanks for taking this on. I have everything in my lab necessary to test
> on E.coli and fungi. My suggestion is to start with E.coli because it's
> very easy to transform and we will be able to tell if the basic design has
> a chance of working.
>
> Below was the lowest cost electroporation device I could find new, but
> they didn't know if it would work on fungi.
>
> http://www.tritechresearch.com/CG-2.html
> On May 1, 2016 2:27 AM, "John Griessen" <john at industromatic.com> wrote:
>
>> On 05/01/2016 12:04 AM, Keoni Gandall wrote:
>> > Just make sure if you send one that there are lots and lots of safety
>> instructions included (I only have basic safety knowledge
>> > due to not being able to take any classes on electronics).
>>
>> Oh, yes. It will be marked.  Maybe I'll figure a way to put a safety
>> switch on yours too! :-)
>>
>> The idea of a safety switch is it disables the danger when the lid is
>> open to expose the wires
>> that contact the cuvette electrodes.  I could put some kind of hinged lid
>> with a limit switch
>> that closes when the lid is down and weighted down enough to push the
>> switch.
>>
>> Then the test procedure would be:
>> 1. load cuvette with xx volume of sample
>> 2. measure resistance of loaded cuvette with Fluke DVM.
>> 3. put cuvette in clothespin holder rig up.
>> 4. close lid and add rock on top.
>> 5. issue commands at python interpreter command window to setup duration
>> and number of pulses.
>> 6. issue python command to fire.
>>
>> Without a safety switch, the commands would be:
>> 1. unplug wall wart AC-2-DC converter.
>> 2. load cuvette with xx volume of sample
>> 3. measure resistance of loaded cuvette with Fluke DVM.
>> 4. be sure 10 seconds has elapsed since unplugging.
>> 5. put cuvette in clothespin holder rig up.
>> 6. close lid and add rock on top.
>> 7. plug wall wart AC-2-DC converter into electroporator.
>> 7. issue commands at python interpreter command window to setup duration
>> and number of pulses.
>> 8. issue python command to fire.
>>
>
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