[Open_electroporator] promoting culture shock progress

[Mitchell Altschuler]

The e.coli test for transformation is rather easy to do if you have a wet lab and I would think is what you really want to test.  Moving dye around is nice to help see whats going on, but that is not the final goal .. 

I only got Will's unit this weekend and hope to learn more about it in the next few days... 

However after just a quick glance,  I am surprised that the capacitors used in this unit are so small ... other designs in the literature older papers use much larger capacitors....but I have  to catch up on how the design is similar or different to other commercial units... 

In those older paper they start with  a 500v power supply .... then just add in the capacitor and a SCRs etc.  in our case I see what looks like a transformer .... and I have to see how all the components function in this device. 

Thank for adding me 

Mitchell 

  

Sent from my new iPhone 7
............…ᘛ⁐̤ᕐᐷ     

> On Jul 10, 2017, at 10:46 AM, Nathan McCorkle <nmz787 at gmail.com> wrote:
> 
> I've never seen bubbles during electroporation. With the original program, I could hear an audible high pitch tone/warble. The latest program I wrote and is on Github is higher frequency pulse spacing (on the coil, not the cuvette), and I couldn't hear anything with it. 
> 
> Sounds like I need to do more testing soon. I wonder if we can come up with a non DNA test that is quicker to pass/fail and iterate. Maybe food coloring or some dye, shock the mix, rinse cells, then check under optical microscope, etc... Maybe I just am thinking too lazy and simply need to devote a day to some full experimentation.
> 
> -Nathan
> 
>> On Jul 10, 2017 7:43 AM, "John Griessen" <john at cibolo.com> wrote:
>>> On 07/10/2017 05:08 AM, Mitchell Altschuler wrote:
>>> If its working I would assume that we should see some bubbles form.
>> 
>> It only applies ~2000 volts for 5 milliseconds.
>> The total power delivered into 30k Ohms, (If that is the resistance of the cuvette with slightly conductive contents),
>> is about 120 Watts, but only for 5 msec.
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