[Open_electroporator] culture shock prototype from Mitchell testing

[John Griessen]

On 07/25/2017 06:57 PM, Nathan McCorkle wrote:
> for e.coli 1800 volts is the default across a 1 mm cuvette.


OK.  Here are parameters you could use on separate loadings of the cuvette to see if
some survive better and if the volt threshold of poration is reached:

First load your cuvette with a suspension of cells in water, measure resistance with a DVM from one side of the cuvette
to the other aluminum metal contact side.  It should be something like 1 or two mega Ohms.  That's what I get with
2 grains of salt in a cuvette full.

Plug in the culture shock to USB, start your terminal emulator and see a prompt >>

Measured with Mitchell's culture shock with no load:
Simple program does pulses then stops, so exp up quick, exp down depends on load, no flat top.
1600V peak:
 >> a(70,5,35)
 >> pulse()

reload cuvette, save and mark that cell suspension [a(70,5,35) 1600V 2mm gap]

1800V peak:
 >> a(70,5,37)
 >> pulse()

reload cuvette, save and mark that cell suspension [a(70,5,37) 1800V  2mm gap]