[Open_electroporator] ask instead *how small of an electroporation cuvette or interelectrode distance could we have?

John Griessen john at cibolo.com
Mon Jan 8 21:33:27 UTC 2018


On 12/29/2017 10:16 AM, John Griessen wrote:
> On 12/28/2017 09:10 PM, Nathan McCorkle wrote:
>> ask instead *how small of an electroporation cuvette or interelectrode distance could we have? To easily discriminate 18kV/cm vs 
>> 18.1kV/cm*
>> we'd need a *58.5 micron* distance. 

Before, I said switching HV is costly so skip it.  I just thought of how we can make a careful software control
of the many switches available in the MCU so as to power down the HV, then throw a switch (that cannot withstand switching
under load with high volts across it), then ramp up the volts with a new measuring bridge of different resistors,
or a new parallel load resistor.

Then three is the "manual way" -- provide finger movable switches engineered for HV, but inexpensive,
and they are safe because they are under the safety cover and all is off and bled down as the cover opens.

So, later on, this can be a tool for delivering small voltage zaps and instrumenting them as in the micro micro scale
of flow cytometry, lab-on-a-chip, low cost field diagnostics creation, etc.


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