[Open_electroporator] culture shock docs -- culture_shock/LAB_MANUAL.md

[Nathan McCorkle]

Overfill is definitely safer in that sense, and easier to spec in a short
sentence. It will just mean some cells and DNA were wasted. Lower
efficiency would but higher predictability means likely overall higher
efficiency in the long run (maybe even one fully wasted cuvette/prep would
be worth it...).

On Wed, Jun 6, 2018, 2:26 PM John Griessen <john at cibolo.com> wrote:

> On 06/06/2018 03:42 PM, Nathan McCorkle wrote:
> > So they're going to realize overfill or over dilution is something to
> avoid and work around.
> >
> > We can also be more specific
>
> underfill is also trouble until we get feedback control of volts working
> well when cuvette
> R varies because of fill level.  I am writing this up as target is fill to
> exact cuvette top volume
> or 10% over and not under.  We can update it again when the feedback
> feature is tested good.
> _______________________________________________
> open_electroporator mailing list
> open_electroporator at cibolo.us
> https://cibolo.us/mailman/listinfo/open_electroporator
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://cibolo.us/pipermail/open_electroporator/attachments/20180606/6b4eb0f4/attachment.html>