[Open_electroporator] low cost bacterial non-salty electroporator proposal

[Nathan McCorkle]

Here are tables of voltage settings for various organisms...
http://m.imgur.com/a/swOXq


Also from this BioRad doc, pg 28

http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006174B.pdf

(screenshot here too) http://imgur.com/TmwPOho

(I would have copied the text, but the PDF is locked and I'm on my work
laptop)

Most Interestingly: E.Coli 12-18kV/cm, test at 5msec pulse, adjust from
that starting point for efficiency tuning

                            S.cerevisieae  ~7.5kV/cm, start at 5msec pulse


On Jun 3, 2016 7:44 AM, "John Griessen" <john at industromatic.com> wrote:

> I'm back to busy working on learning node.js server install of ghost
> blogging and other responsive/trendy website
> function on my new server.  It will help me sell product, use twitter
> effectively, sell service, etc.
>
> I'll take apart one of the little transformers, measure wire size and
> respond to the chinese transformer maker
> to get a quote.  They're better at communicating than most mainland
> Chinese, so maybe there's an easy
> path to using product parts from the start instead of any junk built
> modules for testing.  There IS a need to
> get knowledge of voltage required to get good transformation efficiency in
> a particular size cuvette.  All
> the past cuvettes data, and maybe some info from tritechresearch.com's
> Dr. Papp will help.  The question is
> what max voltage?  3kV?  3500 Volts? 5kV?  For Nathan's future
> microfluidic scale electroporation chambers
> maybe 400 Volts for 15 microseconds?
>
> John
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