[Open_electroporator] low cost bacterial non-salty electroporator proposal

[William Beeson]

I will pre-order one or more and am willing to do some of the biology
prototype testing.  You could use the testing results however you like.  I
would be interested to publish the experience of testing it (once it is
working well) on my website.  We are also building other things like
shaking incubators, homogenizers, etc and making a lot of use of 3D
printing so it would fit in well with that stuff.  If you would rather keep
any testing of it limited to this mailing list that's fine too.

Once I got a hold of Andrew Papp he was very nice and informative on the
phone.  Seemed interested in the use case I was describing and was very
knowledgeable of all the inner workings of electroporation and the settings
etc for use on various organisms.

On Fri, Jun 3, 2016 at 1:49 PM, Nathan McCorkle <nmz787 at gmail.com> wrote:

> Here are tables of voltage settings for various organisms...
> http://m.imgur.com/a/swOXq
>
>
> Also from this BioRad doc, pg 28
>
> http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006174B.pdf
>
> (screenshot here too) http://imgur.com/TmwPOho
>
> (I would have copied the text, but the PDF is locked and I'm on my work
> laptop)
>
> Most Interestingly: E.Coli 12-18kV/cm, test at 5msec pulse, adjust from
> that starting point for efficiency tuning
>
>                             S.cerevisieae  ~7.5kV/cm, start at 5msec pulse
>
>
> On Jun 3, 2016 7:44 AM, "John Griessen" <john at industromatic.com> wrote:
>
>> I'm back to busy working on learning node.js server install of ghost
>> blogging and other responsive/trendy website
>> function on my new server.  It will help me sell product, use twitter
>> effectively, sell service, etc.
>>
>> I'll take apart one of the little transformers, measure wire size and
>> respond to the chinese transformer maker
>> to get a quote.  They're better at communicating than most mainland
>> Chinese, so maybe there's an easy
>> path to using product parts from the start instead of any junk built
>> modules for testing.  There IS a need to
>> get knowledge of voltage required to get good transformation efficiency
>> in a particular size cuvette.  All
>> the past cuvettes data, and maybe some info from tritechresearch.com's
>> Dr. Papp will help.  The question is
>> what max voltage?  3kV?  3500 Volts? 5kV?  For Nathan's future
>> microfluidic scale electroporation chambers
>> maybe 400 Volts for 15 microseconds?
>>
>> John
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>>
>
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