[Open_electroporator] Culture Shock transformer tests

[John Griessen]

On 06/16/2016 02:21 PM, Nathan McCorkle wrote:
> That seems like a test that could be done at lower voltage too, right
> (overlapping/combining pulses to draw them out

There IS a low voltage side way to get everything done with one transformer,
but it does not save you parts in other stages, and that would mean more heat in
the transformer, so I'm going to start out with a half wave low volt switcher, (only NPN
transistor drive), which feeds the transformer with pulses in one direction, and
that feeds a half wave multiplier, which has a polarity.

The other way I ran across while reading is with a full inverter driven by NPN and PNP transistors
(to go to +28V and -28V), and with + and - multipliers and just one transformer.

With the half wave system, the parts count starts low and you can quit while you're ahead enough...
and if you're not quite enough, you can add another identical circuit section and 2 diodes
sum their outputs, (minus 1.3 Volts or so out of thousands), and the timing can be micropython controlled.  If some
observation proved that ripply voltage was bad for electroporation performance, you could add a
third pulser and overlap them with lower duty cycle for each.  I'm guessing two overlapped will be
truly fine, and one, (with voltage going down to zero half the time), will probably be OK
for finding a recipe with decent efficiency.  Think multiple lightning strikes.  Time will tell.