[Open_electroporator] low cost bacterial non-salty electroporator proposal

Nathan McCorkle nmz787 at gmail.com
Mon Jun 20 17:28:11 UTC 2016


On Mon, Jun 20, 2016 at 10:25 AM, John Griessen <john at industromatic.com> wrote:

>>
>> In order to test the device in the real world, I can talk with some friend
>> who works electroporating chicken eggs and zebrafish. I
>> can perform bacteria test by myself.
>
>
> The chicken and zebrafish might work OK if not too salty -- later on I will
> figure how to deal with mammalian
> cell suspensions.

Usually these kind of preparations require less voltage/distance...
much less than E.coli (5-10X less roughly if my memory is correct). I
don't remember them being appreciably more salty, but I've not
researched those cell-prep protocols.



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