[Open_electroporator] Culture Shock yardboard testing

[Nathan McCorkle]

On Jun 21, 2016 9:15 PM, "John Griessen" <john at industromatic.com> wrote:
>> I'd also like to see a screenshot where the pulse generator has more
delay between pulses, so we can really see some dead time
>> padding the edges.
>>
>
> Not sure what "dead time" means.   The ringing won't go away for maybe 5X
the cycle time I showed there.

More time between pulses of the same pulse-width you're showing being used.
So the coil voltage would return to a steady 0V.

> I'll show you some more waveforms tomorrow.  55% duty cycle is what I'm
interested in.  That can be overlapped
> from 2 pulsers diode OR'd for
> continuous summed voltage with some ripple.

I can't remember the "time constant"s (overall pulse length when using
traditional capacitor bleed-down electroporation) that are common for
things like E.coli to think about how many of such pulses we'd need to
overlap. I will try to dig that up, I feel like I've seen a table/graph
with transformation efficiency vs time constant vs field-strength.

I am excited to see pulses being composed of PWM too, once you get an MCU
connected to the transistor, for trying to reduce high-side voltage. Can
you recommend a code compatible MCU I can buy to program pulse generation
stuff here on my end (I know it's micropython but better to be as close to
your setup, and those ST boards are often only $13, right)? I can look into
the hardware timers that connect (via internal muxes) to GPIO for PWM...
unless you've got that covered (I have plenty to do here otherwise as far
as projects go).
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