[Open_electroporator] Culture Shock yardboard testing

[Nathan McCorkle]

On Jun 27, 2016 9:03 AM, "John Griessen" <john at cibolo.com> wrote:
>
>
>
> On 06/27/2016 10:28 AM, Nathan McCorkle wrote:
> >> On Jun 27, 2016 7:46 AM, "John Griessen" <john at industromatic.com>
wrote:
> >> Yes the all purpose one you used was able to do that huge power
transfer, and it had
> >> a huge box
> >
> > No actually, it was quite small, equivalent if not less than a toaster
in volume (compared to other  electroporation units it was
> > small). Also I don't care if the box is huge, more that it works and is
effective and obtainable for others.
>
> I do care about such things because they are crude and won't make a
viable product.
>

I mean I literally don't care about talking about such things, as
personally I don't think they matter one bit from a potential sales
point... people I see as disadvantaged need function and price point. Of
course smaller is better, we are living in the time of Moore's law. Talking
about this just seems like a waste of time, as isn't smaller is better
assumed in this space? I mean, no one has ever asked for a BIGGER
electroporator... so why do you even bring it up?


My reason for posting references to LARGER and HEFTIER products was not the
point, the point was their purpose was for delivering lots of current in a
quick pulse. I ASKED if a topology like this could be utilized with SMALLER
components, because I KNOW our target application is MUCH less intensive.


> Another thing about the biorad gene pulser type of discharge system is it
puts its own load resistor in parallel with the sample,

> which keeps the volt curve uniform, but needs a lot of extra power flow,
instead of it all going into the
> cuvette.

Ok, I will think about that one a bit more, makes sense at first go though.

> We are designing an instrumented system instead of a blind one, so we can
see volt curves, then see results,
> and decide to change the amount of time or the voltage we subject the
cuvette to.  Very different, much smaller
> power needs, and makes a viable product.  Toaster sized is what I call
huge, and not a goal.
>
> You seem to be getting squeamish about my time spent and results.  If
you'd like your money back that's fine, can do.


No, it actually has nothing to do with results and time spent, it is
completely due to my interpretation and feeling that my questions and
discussion points went unheard and ignored, and ultimately unaddressed.
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