[Open_electroporator] Pragmatic electroporation protocols

[John Griessen]

On 06/27/2016 04:21 PM, Keoni Gandall wrote:
> the slow-down that cold provides likely doesn't outweigh the survivability costs, as more healthy cells will survive electroporation.

So, do cells survive better warm?  Warmth increases chance of arcing said one of the pages at tritechresearch.com.

There is a chance that with a micropython controlled volt profile (like was never available before), a lower voltage for a little 
longer and maybe tapering off some will get good results warm.  Capacitive discharge pulses are exponential up/down, so they I bet 
they start arcing right away based on the early peak of such a pulse.  Letting the volts and thus the e field build up to a flat 
top for a millisecond or 7 could be good for not arcing -- When the volt level is just right.