[Open_electroporator] Pragmatic electroporation protocols

[Nathan McCorkle]

On Mon, Jun 27, 2016 at 2:21 PM, Keoni Gandall <koeng101 at gmail.com> wrote:
> Overall, if you are making your own electrocompetent cells, perhaps try to
> do it at room temperature instead of rushing around with ice. I know that's
> wayyyyy easier for me in a DIY setting.

I've done it warm a couple of times, just using cells that were
subcultured ~6 hours before, then pelleted and resuspended in water a
few times before adding plasmid DNA and zapping with the MicroPulser.
I have also done it cold, but those times I was using
"electrocompetent" cells which were rinsed with much finer fluids than
plain water... so they had higher efficiencies, but I have never had
arcing in any case (though I can't quite remember what happened when I
tried using a grill lighter to electroporate, heh heh).